Andalusia - The Alchemist Allusions Jairath, V. & Feagan, B. G. Global burden of inflammatory bowel disease. Grzelczak, M., Liz-Marzn, L. M. & Klajn, R. Stimuli-responsive self-assembly of nanoparticles. Google Scholar. Dong, A., Chen, J., Vora, P. M., Kikkawa, J. M. & Murray, C. B. Binary nanocrystal superlattice membranes self-assembled at the liquid-air interface. 1f). Nanoparticle assembly has been proposed as an ideal means to program the hierarchical organization of a material by using a selection of nanoscale components to build the entire material from the bottom up. 25 Nov 2020 Vol 6, Issue 48 DOI: 10.1126/sciadv.abb8675 Abstract Electrostatic interactions between nanoparticles (NPs) and functionalized ligands lead to the formation of NP surfactants (NPSs) that assemble at the water-oil interface and form jammed structures. Mater. Baik lingkungan alam, ekonomi dan politik. Nano Lett. P.J.S., P.A.G. Mater. 50, 48084842 (2011). Nanoparticle assembly and attachment are common pathways of crystal growth by which particles organize into larger scale materials with hierarchical structure and long-range order. The authors declare no competing interests. Facile fabrication of a single-particle platform with high throughput Open Access h No significant differences in body weights were observed between the rats receiving oral administration of NPA2 coacervate on days 1, 3, 5, 7, and 9, and untreated healthy rats during the 10-day toxicity evaluation. Crystal structure, Materials, Nanoparticles Nanoparticle assembly enables the synthesis of multiple complex nanoscale structures, but future investigation and application of these materials requires new processing methods to control micro- and macroscale structure as well. Finally, sections were stained with hematoxylin. However, creating such ordered complex structures using synthetic nanoparticles is a challenging task and requires a high degree of control over structure and multiple non-covalent interactions. The total dosage of Dex-P administered in the two groups was the same (~1.15mg). Chem. b Immunohistochemical staining against key macrophage M1/M2 polarization markers iNOS/CD206, c histopathology score, d mRNA levels of tight junction-associated protein ZO-1, e pro-inflammatory cytokines including interleukin IL-1 and IL-6 released by M1 macrophages, and f anti-inflammatory cytokine IL-10 released by M2 macrophages. Nature 591, 586591 (2021). Mater. J. The staining was further developed using the Vectastain ABC Kit (Vector Laboratories) and the DAB (3, 3-diaminobenzidine) Substrate Kit (Vector Laboratories) for peroxidase. Article Love, C. et al. Adv. designed the experiments and wrote the manuscript. It contains Supplementary Figs. 12, 11651173 (2020). Article [2306.15617] Toward a unified description of the electrostatic assembly PubMedGoogle Scholar. Kang, H. et al. These authors contributed equally: Pengchao Zhao, Xianfeng Xia, Xiayi Xu. f Dex-P-laden NPA2 coacervate delivered by oral gavage better sustained the serum Dex concentration at the lower therapeutic level compared with that of the oral gavage of Dex-P aqueous solution (Dex-P/PBS) in vivo. Ni, J., Wu, G. D., Albenberg, L. & Tomov, V. T. Gut microbiota and IBD: causation or correlation? For in vivo Dex-P release, healthy SD rats or DSS-induced colitic rats (200300g, female, 812 week) received a single oral gavage of 1.0ml as-prepared Dex-P-laden NPA2 coacervate (Dex-P/NPA2) or Dex-P aqueous solution (Dex-P/PBS). Soc. 17). 6, 527 (2014). 4d, fi. Taken together, oral administration of Dex-P/NPA2 to colitic SD rats promoted the M2 polarization of intestinal macrophages61 and mediated strong anti-inflammatory effects against acute colitis, thereby creating a favorable immune microenvironment to promote colon repair and regeneration (Fig. 14, 573 (2017). P.Z., X.X., X.F.X., and B.Y. Chem. Our NPA2 coacervate showed a catechol oxidation potential (E0) of 0.596V in deionized water, which was significantly higher than the E0 of soluble small molecule 4-methylcatechol (0.455V)57. Mirkin, C. A., Letsinger, R. L., Mucic, R. C. & Storhoff, J. J. S10) or 18 wt % as shown by small-angle X-ray scattering (SAXS) measurements. The color of the square shows the distance of evolution between each two samples. pH-Driven Reversible Assembly and Disassembly of Colloidal Gold Finally, the analyses of air-dried samples were performed in an AutoFlex Speed LRF MALDI-TOF mass spectrometer (Bruker Daltonics, Germany) under reflector mode. Block copolymer/nanoparticle films were prepared by spin-coating toluene solutions of a mixture of 3- or 5-wt% polystyrene- block -poly (2-vinylpyridine) copolymer, denoted PS- b -P2VP, and 1-wt%. Adv. It should be noted that electrostatic interactions between polyanions and polycations weaken with increasing salt concentrations; therefore, a critical salt concentration of 0.82.0M NaCl generally can lead to the rapid dissociation of complex coacervates19,22. Auyeung, E. et al. Mater. Macroscopic materials assembled from nanoparticle superlattices Department of Mechanical Engineering, The University of Hong Kong, Hong Kong SAR 999077, China . In contrast, the NPA3 coacervate demonstrated a much lower viscosity than that of the NPA2 coacervate, and this can be attributed to the weak interaction energy of interactions (<10kJmol1) (Fig. For in vitro Dex-P release, 2ml of Dex-P in PBS (1.5mg/ml, TCI) was added to 1ml NPA2 coacervate followed by vortex for 15s for Dex-P encapsulation. Mussel-mimetic hydrogels with defined cross-linkers achieved via controlled catechol dimerization exhibiting tough adhesion for wet biological tissues. We have also tried to treat DSS-induced colitic rats with the equivalent amount of Dex-P (1.15mg, MCE, USA) in PBS (Dex-P/PBS) via enema on days 1, 3, and 5. Singh, G. et al. Med. Scale bar for immunohistochemistry staining, 50m. 2). Mater. [2] The small size of nanoparticles allows them to have unique characteristics which may not be possible on the macro-scale. Precise micro-channels and channel inlets effect the flow, allowing for control over the self . 4f). Robert J. Macfarlane. CEN Solutions hiring Delineante mecnico in Dos Hermanas - LinkedIn PubMed Immunohistochemistry staining against CD206 (M2 marker) or iNOS (M1 marker) showed increasing M2 macrophage polarization in the colitic SD rats receiving Dex-P/NPA2 (Fig. J. Self-assembly of colloidal nanoparticles into 2D arrays at water-oil The nanoparticle assembly strategy may be further refined with alternative polyanions and surface stabilizing agents; it may also be applicable to other polycationic antibiotic candidates that encompass a wide range of antimicrobial peptides [50]. 38, 213226 (2019). Soc. All data are available in the main Article and Supplementary Information, or from the corresponding author upon reasonable request. Cancer 117, 15831591 (2017). Rose, S. et al. Nature 466, 474477 (2010). Adv. Although many forms of oral drug delivery vehicles have been developed, the insufficient residence time within the intestinal tract and the complex design of delivery vehicles limit their widespread clinical applications7,8,9,10. High-performance mussel-inspired adhesives of reduced complexity. *p<0.05, **p<0.01, ***p<0.001 (Ordinary one-way ANOVA). Programmable Nanomaterials - Mirkin Nanoparticle assembly mechanisms within biobased polymer matrices were probed using in situ liquid-phase atomic force microscopy (AFM) and computational simulation. et al. Huh, J.-H., Lee, J. Tang, T. D. et al. J. Br. Mater. Vass, P. et al. Clinical manifestations of the colitis, such as severe rectal bleeding, watery diarrhea, and colonic edema were observed after 7 days (Supplementary Fig. 197-203 (Springer, 2016). Talapin, D. V., Lee, J.-S., Kovalenko, M. V. & Shevchenko, E. V. Prospects of colloidal nanocrystals for electronic and optoelectronic applications. Ahn, B. K., Lee, D. W., Israelachvili, J. N. & Waite, J. H. Surface-initiated self-healing of polymers in aqueous media. Liu, S. et al. Get the most important science stories of the day, free in your inbox. Then 2ml fresh PBS buffer (1, Gibco) was added to the as-prepared Dex-P-laden NPA2 coacervate (1ml). Surgical materials: current challenges and nano-enabled solutions. Sprague Dawley (SD) rats with unrestricted access to water and standard laboratory diet received a single oral gavage of 1.0ml NPA2 or NPA3 coacervate (modified with a Cy7 tag, a near-infrared fluorescent dye) and were sacrificed after 0, 6, 24, or 48h to evaluate the fluorescence retention in the harvested GI tract (Fig. PubMed Glucocorticoid-induced leucine zipper is protective in Th1-mediated models of colitis. Programmable atom equivalents: atomic crystallization as a framework for synthesizing nanoparticle superlattices. Posted 10:00:15 AM. Nat. Hepatol. Dex-P concentrations in the above PBS were measured at 242nm. & Puntes, V. Kinetically controlled seeded growth synthesis of citrate-stabilized gold nanoparticles of up to 200 nm: size focusing versus Ostwald ripening. Adv. 5). GPC analysis showed that no degraded or dissolved NPA2 coacervate components were found in the simulated body fluids (Ga, In) after 3 days of incubation (Fig. 5b and Supplementary Fig. Nature 440, 319323 (2006). Liu, J., Tan, C. S. Y. We confirmed the above hypothesis by examining the cyclic voltammetry (CV) curve of the NPA2 coacervate (Fig. An inflammation-targeting hydrogel for local drug delivery in inflammatory bowel disease. Li, F. et al. Immunofluorescence staining for tight junction-associated proteins was performed using antibodies against ZO-1 (Santa Cruz, sc-33725) and occludin-1 (Santa Cruz, sc-271842), respectively. Na, Y. R., Stakenborg, M., Seok, S. H. & Matteoli, G. Macrophages in intestinal inflammation and resolution: a potential therapeutic target in IBD. Appl. The NPA2 coacervate also remained largely stable in the 25mg/ml pig bile salt or under the high shear strain (up to 1000%) despite a slight dip in shear moduli toward the high shear range (Supplementary Figs. Liquid-metal nanoparticles were used as reservoirs for mixing with various metal salts (left). & Estournes, C. Sintering and densification of nanocrystalline ceramic oxide powders: a review. Source data are provided as a Source Data file for Fig. About 5mg/mL NPA2 coacervates were used to culture the cells for 7 days. 7). Immunohistochemical staining for macrophage polarization was performed using an antibody against CD206 (M2 marker, Abcam, ab64693, dilution of 1:200) or iNOS (M1 marker, Abcam, ab283655, dilution of 1:200), followed by Vectastain ABC Kit (Vector Laboratories) and the DAB (3, 3-diaminobenzidine) Substrate Kit (Vector Laboratories). ACS Nano 5, 60816084 (2011). Stereol. Source data are provided as a Source Data file for Fig. Nano Today 9, 574589 (2014). 144, 158 While the conventional crossover tile strategy requires a two-step process of tile construction followed by nanoparticle assembly, DNA origami utilizes a simple and versatile 'one-pot' process to induce the folding of long genomic DNA scaffolds into . Organically linked iron oxide nanoparticle supercrystals with exceptional isotropic mechanical properties. a-c, Assembly.d,e, Disassembly.a, Nanoparticles smaller than the blue block's R 0 are miscible and assemble into a lamellar structure. Nanoparticle solutions as adhesives for gels and biological tissues. Sang, X. et al. 19, 118126 (2019). High-Strength Magnetic Hydrogels with - ACS Publications In conclusion, we have developed a simple yet highly efficient strategy for oral drug delivery by using a nanoparticle-assembled (NPA) bioadhesive noncomplex coacervate. Finally, a Multiskan microplate reader (Thermo Fisher) was used to collect the absorbance value of each well at 540nm for cell metabolic calculation. Int. Macrophage-targeted lung delivery of dexamethasone improves pulmonary fibrosis therapy via regulating the immune microenvironment. Besides, our laboratory-scale synthesis yielded ~300ml of NPA2 coacervates per batch and showed stable batch-to-batch reproducibility, indicating the promising potential for large-scale production of NPA2 coacervates.